Isolation and identification of chemical constituents from Disporum cantoniense (III) / 中草药

Objective: To study the chemical constituents from Disporum cantoniense. Methods: Separation was carried out by ion exchange chromatography, medium pressure MCI column chromatography, ODS column chromatography, gel column chromatography, preparative and semi preparative liquid chromatography; The structures of the compounds were identified by modern spectral techniques such as mass spectrometry and nuclear magnetic resonance. Results: Eleven compounds were isolated and identified as 2’-β-D-glucopyranosyloxybenzyl-6-α-L-(4’-O-acetyl)-rhamnopyranosyloxy-2-hydroxy-3-methoxybenzoate (1), 4’,7- dihydroxyflavone (2), palmatine (3), marmesinin (4), 4’-O-β-D-glucosyl-5-O-methylvisamminol (5), nodakenetin (6), 2-aminopyridine (7), tenuifoliside A (8), neosakuranin (9), 2″-O-rhamnosylicariside II (10) and baohuoside I (11). Conclusion: Compound 1 is a new compound named disporumoside, compounds 2-11 are isolated from the genus of Disporum for the first time.

Imaging classification diagnosis and maxillary sinus floor augmentation of maxillary sinus cystic lesions / 华西口腔医学杂志

Maxillary sinus cystic lesions can often be found in cone-beam computed tomography (CBCT) images. However, whether this change affects the implementation of maxillary sinus floor augmentation remains unclear. Combining the common cystic change performance of CBCT, image classification diagnosis of maxillary sinus cystic change was introduced, and the indications and surgical methods of maxillary sinus floor augmentation and postoperative radiographic changes of mucous were analyzed. This procedure may help clinicians evaluate the feasibility and methods of maxillary sinus augmentation in maxillary sinus cystic changes.

Spine and extremities impairment: comparative study on clause for Identification of the body injured and Classification of the body impairment / 中国法医学杂志

The article is comparative study about spine and extremities on clause for the identification of the body injured and The classification of the body impairment. We reviewed the terms and provisions about spine and extremities as follows, amputation impairment, function impairment, and amputation impairment combined function impairment. This paper provides a comprehensive access and analysisofthe similarities and differences between the two standards.

A study on detection of α-helix protein in posttraumatic epileptogenic focus by FTIR-mapping / 中国法医学杂志

Objective The article is to study on the detection of α-helix proteins in post-traumatic epileptogenic focus by FTIR-mapping. Methods Fourier transform infrared spectroscopy-mapping were applied to identifying α-helix by point-by-point scanning in post-traumatic epileptogenic focus sections and to develop FTIR-mapping profiles. Result The high absorbance of α-helix is accord with post-traumatic epilepsy, there are some significant differences between high absorbance and low absorbance. Conclusion α-helix proteins are distributed in post-traumatic epileptogenic focus widely, thus α-helix protein are involved in post-traumatic epilepsy.

Graphical modeling of ICF Core Set (Comprehensive Version for Stroke) / 中国法医学杂志

Objective It aims to investigate the relationships among the categories of Comprehensive Version for Stroke as described in the International Classiifcation of Functioning, Disability and Health (ICF) Core Set, and to provide new supports for Judicial Appraisal of functioning in stroke by ICF functioning mapping.Methods The variables of 59 categories of ICF assessment scale and the samples of 106 persons’ are selected and used in the least absolute shrinkage and selection operator (LASSO) for mining dependencies among those variables. The graphical modeling and analyzing with the software Gephi provides a visual map of the correlations among those classiifcations. Results 59 interconnected categories which organized into the functioning mapping. b340, b735, b175 and b152 are centrally positioned categories because of their high correlation.Conclusion Functioning mapping by graphical modeling can reveal complex relational structures embedded in functioning classiifcations, which provides the support for using ICF to appraisal stroke.

Correlation between postmortem intervals and the changes of 18s rRNA degradation in liver after death under the ;condition of different temperature( / 中国法医学杂志

Objective To investigate the relationship between Ct value of mice liver and postmortem interval (PMI) under various ambient temperatures. Methods mice were stored at 10℃, 15℃, 20℃, 25℃ and 30℃ after execution, and total RNA was extracted from mice liver every 6 hours (PMI 6h to 72h). The levels of 18s rRNA were examined using real-time PCR. The results were expressed by cycle threshold (Ct) value to explore relationship between PMI and Ct value, and the interpolation functions were established to estimate PMI. Results In each group, Ct value increased with PMI increased. Surface equation was obtained after interpolation analysis on temperature range 10℃~30℃. The three-variable quintic surface equation was f(x, y)=-426.9+30.82x+44.48y-1.297x2-1.837xy-1.388y2+0.034 38x3+0.038 17x2y+0.038 67xy2+0.028 77y3-0.000 612 9x4-3.897e-7x3y-0.001 223x2y2+0.000 256 6xy3-0.000 537 4y4+3.606e-6x5-2.846e-6x4y+1.009e-5x3y2-3.439e-6x2y3-2.556e-7xy4+2.664e-6y5(r2=0.999 4). Conclusion The rule of Ct value changes at ambient temperature complied with three-variable quintic surface equation distribution. Measurement of interpolation function may be used for PMI estimation at ambient temperature.

Cone-beam CT features of ameloblastomas / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To summarize and analyze the cone-beam CT (CBCT) imaging features of ameloblastomas for clinical preoperative diagnosis.</p><p><b>METHODS</b>Thirty-seven cases with complete data proven by pathology were retrospectively studied for distinct CBCT features.</p><p><b>RESULTS</b>Of the 37 cases, 31 were primary and 6 were recurrent. On CBCT images, the 37 cases were composed of 36 cases (97.3%) of intraosseous lesions (17 cases of multilocular lesions, 17 cases of unilocular lesions, and 2 cases of honeycomb lesion) and 1 case (2.7%) of extraosseous lesion (soft tissue lesion). Of the 17 multilocular cases, 15 (88.2%) showed tongue-shaped crests in lesions. Thirty-four cases (94.4%) showed apparent expansible change toward lip/buccal and (or) palatal/lingual sides ofjaw, with partialcortical erosion.</p><p><b>CONCLUSION</b>CBCT imaging yields accurate three-dimensional images of lesion shape and structure. Therefore, CBCT can be usedfor the clinical diagnosis and surgical assessment of ameloblastoma.</p>

Cone beam CT radiographic diagnosis of submandibular radiopaque sialolithiasis / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the radiographic features of submandibular sialiths in cone beam CT (CBCT) images.</p><p><b>METHODS</b>Eighty-four patients with submandibular radiopaque sialiths were included in this study. The clinical features of gender and age and the radiographic features on CBCT, including the number, morphology, size, and location, were recorded for further statistical analysis.</p><p><b>RESULTS</b>A total of 128 sialiths were detected from the 84 subjects; 22 subjects (26.19%) had multiple sialiths. The morphology of the sialiths was classified into five types: spot, round, spheroid, elongated, and irregular shapes. Among these types, the spheroid shape was the most frequently detected. A correlation was observed between the size of the sialiths and their location, with the large sialith located at the posterior portion of the duct. About 39.06% (50/128) of sialiths was located at the anterior portion of the duct, and 60.94% (78/128) was located at the posterior portion. The horizon- tal position of the sialith was significantly correlated with its vertical position (P < 0.0001).</p><p><b>CONCLUSION</b>The CBCT images showed important data for the evaluation, diagnosis, and treatment plan of the submandibular sialolithiasis.</p>

Analysis of imaging findings in jaw bone islands / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To provide reference data on frequency and distribution of bone islands (BIs) and investigate their relationship with age, gender, and localization.</p><p><b>METHODS</b>A population who received a pretreatment and at least one follow-up panoramic radiograph in the Department of Oral Radiology, West China Hospital of Stomatology, Sichuan University, were selected for this retrospective study. A sample population of 29,556 patients (12,824 males and 16,732 females) with different dental complaints and ages ranging from 8 to 80 years (mean age: 23.95 years) were included.</p><p><b>RESULTS</b>In the radiographic evaluation, BIs appeared as localized, well-defined, non-expansile, radiopaque masses which were round, elliptic, or irregular in shape. Their sizes varied from a few millimeters to about 2 cm in diameter. A total of 598 radiopacities were detected, and 545 patients of 29,556 patients (1.84%) had BIs. Among subjects with multiple lesions, 49 patients had 2 BIs and 2 patients had 3 BIs. The BIs had immense mandibular predilection, with presentation primarily in the premolar/molar region. The condition appeared to have no tendencies based on sex.</p><p><b>CONCLUSION</b>Recognition of BIs variation is significant in dental examinations.</p>

Morphology of the soft palate in normal individuals when pronouncing: a digital cephalometric study / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the morphology of the soft palate in normal individuals with digital radiography, when they pronounced the high vowel of "i", and to provide the references for therapy of the cleft palate.</p><p><b>METHODS</b>In this study, the sample comprised 27 normal subjects. With the digital cephalometry, the morphology of the soft palate when pronouncing the high vowel of "i" was observed. And the dimensional difference of the soft palate when pronouncing between different gender was studied.</p><p><b>RESULTS</b>When pronouncing the high vowel of "i", the morphology of the soft palate was like the shape of the knee. And it could be divided into two parts: horizontal and vertical. The length of the vertical part in male group was (24.92 +/- 2.03) mm, the length of the vertical part in the female group was (20.66 +/- 2.77) mm. The length of the vertical part was different between male and female group (P < 0.001).</p><p><b>CONCLUSION</b>The morphology of the palate when pronouncing the high vowel of "i" is similar. And the velar length of the vertical part of the male is longer than the female.</p>

The effect of prostatectomy on nocturia in patients with benign prostatic hyperplasia / 中华外科杂志

<p><b>OBJECTIVE</b>To explore the effect of prostatectomy on nocturia in patients with benign prostatic hyperplasia (BPH).</p><p><b>METHODS</b>The data of patients who had received prostatectomy for BPH between June 2006 and December 2007 were collected. Nocturia severity was assessed preoperatively and 3 to 6 months after prostatectomy by the number of nocturia events, the time from falling sleep to the first awakening to void (hours of undisturbed sleep, HUS), the score of the nocturia quality of life (N-QOL) questionnaire, the International Prostatic Symptom Score (IPSS) and the quality of life (QOL) score.</p><p><b>RESULTS</b>One hundred and twenty five cases were included. Of them, 73 patients finished the follow-up completely. There were 62 patients whose number of nocturia events before the operation was equal or more than 2. The data from these 62 patients were analyzed. Of them, 56 patients underwent transurethral resection of prostate, the remaining 11 patients suprapubic prostatectomy. Significant improvement (P < 0.01) was noted in all the following parameters after treatment: the number of nocturia events decreased from 4.2 ± 2.4 to 2.2 ± 1.0, HUS increased from (1.8 ± 0.7) h to (3.0 ± 1.4) h, N-QOL score raised from 30 ± 10 to 40 ± 7, IPSS decreased from 23 ± 5 to 8 ± 5, and QOL score fell down from 4.4 ± 0.7 to 1.5 ± 1.0.</p><p><b>CONCLUSION</b>The prostatectomy can markedly improve the symptoms of nocturia, sleep and life quality in the BPH patients who accompanied with nocturia.</p>

Construction of nano-granule titanium film and study of its surface topography / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To construct four different micro- and nano-phase titanium film models and investigate the characteristics of their surface micro-topography.</p><p><b>METHODS</b>Four different titanium films were prepared on commercial titanium discs, by direct current magnetron sputtering, at ambient, 100, 250, 380 degrees C substrate temperature, respectively. Their surface topography and crystal sizes were investigated using atomic force microscope (AFM) and X-ray diffraction (XRD). The size of granule and surface roughness in different group was calculated and compared.</p><p><b>RESULTS</b>All samples were covered by a thin film consisting of dense round or ovaloid granules. The granules and crystals was growing as the substrate temperature increasing. The Ti substrate had greater effect on the surface topography of film compared with Si substrate. This kind of complex topography caused the surface roughness of Ti substrate group decreased as the granules growing.</p><p><b>CONCLUSION</b>In our study, four different micro- and nano-phase titanium film models were constructed for our coming investigation of their topographical influence on biological reaction of proteins and cells. Basic data on surface features was obtained for next in vitro and in vivo experiment.</p>

Construction and immunogenicity of attenuated Salmonella typhimurium stably harbouring DNA vaccine against Newcastle disease virus / 病毒学报

The fusion protein (F) gene of Newcastle disease virus was amplified by polymerase chain reaction (PCR) from the recombinant plasmid pVAX1-F, and subcloned into eukaryotic expression vector pmcDNA3. 1+. The F gene was identified by sequencing. The recombinant plasmid was transformed into attenuated Salmonella typhimurium SL7207, and the recombinant was designated as SL7207 (pmcDNA3. 1-F). In vitro and in vivo experiments showed that the plasmid stability of pmcDNA3. 1-F was apparently higher than that of pcDNA3. 1-F in SL7207. In order to compare the immune response induced by these two re combinant bacteria, BALB/c mice were immunized orally with them at the dosage of 2 x 10(9) CFU respectively. Both SL7207(pcDNA3. 1-F) and SL7207(pmcDNA3. 1-F) initiated F-specific serum and mucosal antibodies in immunized mice. Furthermore, 4-day-old SPF chickens were immunized with SL7207(pcDNA3. 1-F) and SL7207(pmcDNA3. 1-F) at the dosage of 5 x 10(9) CFU and boosted two weeks later with the same dosage. Humoral and intestinal mucosal immune responses were observed and their levels were significantly higher than that of negative and positive controls. The result of protective efficacy showed that the chickens immunized with SL7207(pmcDNA3. 1-F) had the protective rate of 70.0%, higher than that of the SL7207 (pcDNA3. 1-F) with 50.0%. In summary, the DNA vaccine delivered by attenuated Salmonella typhimurium has good immunogenicity. A novel mucosal DNA vaccine has been developed and could be useful for controlling the infection and epidemic of Newcastle disease in the poultry.